微型膜片鉗-時間分辨的低電流檢測儀
作者: Kevin Peng 發(fā)布于: 2016-08-23 17:11 點擊:次
從單個細胞來看, 生命物質(zhì)的特征之一是細胞被一層膜所包圍。我們已經(jīng)知道, 活細胞的結構比較復雜, 其中膜結構實際上涉及到細胞活性的各個水平。例如, 在真核細胞中, 膜充滿了細胞, 出現(xiàn)在線粒體, 高爾基體和細胞核等。這些細胞膜起到把細胞質(zhì)分開并為生命過程的發(fā)展提洪骨架的作用。盡管細胞膜有內(nèi)外之分, 但一般認為它們是互相連通的。人們常把蛋白質(zhì)或核酸等看作基本“ 生命” 實體, 但從更多的證據(jù)來這些細胞要素在某些類型的膜把它們從周圍環(huán)境中分離開以前是不會發(fā)生更多轉(zhuǎn)化作用的。由于許多生理和生物化學作用不是發(fā)生在大量細胞質(zhì)內(nèi), 而是發(fā)生在各種細胞膜中, 因此生物膜便成為生物化學和生物物理學所研究的核心問題之一，這個時候我們就需要膜片鉗。
:膜片鉗技術是在電壓鉗技術的基礎上發(fā)展起來的, 來反映細胞膜上離子通道活動的一項技術。它對通過離子通道的離子電流進行記錄, 目前已發(fā)展出多種記錄模式, 廣泛應用于神經(jīng)科學、藥理學、細胞生物學、生理學和分子生物學等學科領域。
膜片鉗技術(patch-clamptechnique)是在電壓鉗技術的基礎上發(fā)展起來的, 采用記錄流過離子通道的離子電流, 來反映細胞膜上單一的(或多個的)離子通道分子活動的技術。該技術可將一尖端經(jīng)加熱拋光的玻璃微電極管吸附只有幾平方微米的細胞膜, 在玻璃電極尖端邊緣與細胞膜之間形成高阻封接, 因而可通過微電極直接對膜片(細胞膜小區(qū)域)進行電壓鉗制,而無需使用其它微電極(如圖)。1976年, 德國生理學家Neher和Sakmann[ 2] 首先利用此技術研究肌肉細胞膜上的乙酰膽堿受體通道, 記錄出了量值在皮安級(10-12 A)的微弱電流。
基于元素的設計定制芯片(ASIC)使用標準和低成本的CMOS工藝。多年的經(jīng)驗在微電子設計應用于若設備給團隊一個獨特的元素,在非常低振幅的信號讀出獨占優(yōu)勢。元素微芯片可以測量微微安培電流(10 - 12)與帶寬高達數(shù)百千赫范圍內(nèi)由于深知識達到單元素和多通道信號采集非常低振幅信號,低功率管理技術、模擬數(shù)字轉(zhuǎn)換和數(shù)字數(shù)據(jù)精化。
公司開發(fā)了單通道和四通道兩種微電流放大器，廣泛應用于電化學，生物物理，細胞檢測，生物光子學，生理學，納米電極，納米通道 等領域，被業(yè)界認可，被很多膜片鉗的檢測系統(tǒng)廠家采用，具有價格便宜，體積小，便于構建等優(yōu)點，可適用于廣大科研用戶和OEM客戶。
eONE – Miniaturized One-Channel Amplifier
The world’s smallest low-cost current amplifier for nanopores and single-channel electrophysiology applications. eONE is a complete, USB-powered, one-channel data acquisition system. It incorporates the low noise amplifier, the digitizer and filters, and the voltage stimulus generator, all in a very small footprint (only 30x15x74mm). It is suitable for research and educational activities which use bilayer lipid membranes (BLM) or whole cells.
A small, easy-to-use, USB plug ‘n play system for acquiring very low-current signals!
Simple experimental setup
How to connect eONE in a typical electrophysiology setup
Orbit mini - World's only 'hand-held' four-channel bilayer setup
Thanks to the collaboration with Nanion Technologies and Ionera, the Orbit mini, the world's only handheld four-channel bilayer setup, has been developed. The core of the system is eFOUR, the smallest four-channel, USB-powered, current amplifier designed and produced by Elements.
A compact and complete BLM educational kit for accurate electrophysiological recordings. The kit includes the eONE amplifier, the EDR software with real-time analysis and all the tools for fast screening of ion-channels functional activity in lipid environments.
The kit is contained into a 16 x 10 x 5 cm Faraday cage and includes:
• a recording chamber made by two 1 ml Delrin cuvette with electrode input;
• a set of laser drilled Teflon membranes with fixed hole size of 50, 80, 110 µm;
• paint brush, Ag wires, electrode connectors to plug the eONE amplifier.
A large number of standard experiments can be performed using the BLMkit. Stable membranes can be easily formed to study ion channels or toxin property. Without bulky setup you can start to make BLM experiments on your desk. The BLMkit is especially design for teaching activity for lab experience, to enable students to learn electrophysiology basis on Lipid Bilayers property, membrane protein study and ion channels analysis.
EDA - Elements Data Analyzer
The Elements Data Analyzer software enables users to open and visualize the current and voltage traces acquired by Elements Data Reader in the .dat and .abf formats. The intuitive graphical user interface allows a quick and easy data display.
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